Journal: Journal of Applied Biological Chemistry

Article Title: The Acetylation-based synthesis of 3,3',4',5,5',7-hexaacetate myricetin and evaluation of its anti-inflammatory activities in lipopolysaccharideinduced RAW264.7 mouse macrophage cells

doi: 10.3839/jabc.2023.005

Figure Lengend Snippet: Fig. 5 Effects of MYR and MYR-HA on the protein expression levels of COX-2 (A) and iNOS (B) in LPS-stimulated RAW 264.7 cells determined by western blot and quantified using ImageJ. Means not sharing a common superscript are significantly different (ANOVA, p <0.01). Values are mean ± SD. For post-hoc analysis, Tukey-Kramer test was per-formed

Article Snippet: Antibodies against β-actin (cat. no. VMA00048), inducible nitric oxide synthase (iNOS) (cat. no. AHP2399) were purchased from Bio-Rad (Hercules, CA, USA), and anti- Cyclooxygenase-2 (COX-2) antibodies (cat. no. 100-401-226) from Rockland (Limerick, PA, USA).

Techniques: Expressing, Western Blot

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments.

doi: 10.1016/j.biopha.2023.114964

Figure Lengend Snippet: Fig. 3. The effect of SDG on IL-1β-stimulated inflammation and ECM degradation in SW1353. (A)The protein expressions of COL2A1, ADAMTS5, SOX9, MMP13, INOS and COX2 in SW1353 were evaluated by western blotting. (B)The ADAMTS5 were detected by fluorescence microscopy, combined with nuclear DAPI staining (scale bar: 200 µm). (C)The COL2A1 were detected by fluorescence microscopy, combined with nuclear DAPI staining (scale bar: 200 µm).The values are mean ± SD for 3 separate experiments. *p < 0.05, **p < 0.01 and ***p < 0.001. SDG, secoisolariciresinol diglucoside; ECM, extracellular matrix; COL2A1, collagen II; ADAMTS5, a disintegrin and metalloproteinase with thrombospondin motifs 5; SOX9, SRY-related high-mobility-group-box gene 9; MMP13, matrix metal loproteinases 13; INOS, inducible nitric oxide synthase; COX2, cyclooxygenase-2.

Article Snippet: Primary antibodies against Actin, COL2A1, ADAMTS5, SOX9, MMP13, INOS, COX2, Nrf2, HO-1 and Z. Zhang et al. Biomedicine & Pharmacotherapy 164 (2023) 114964 IκBαwere bought from Boster (Wuhan, China).

Techniques: Western Blot, Fluorescence, Microscopy, Staining

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments.

doi: 10.1016/j.biopha.2023.114964

Figure Lengend Snippet: Fig. 4. The effect of SDG on IL-1β-stimulated inflammation and ECM degradation in mouse primary chondrocytes. The protein expressions of COL2A1, ADAMTS5, SOX9, MMP13, INOS and COX2 in mouse primary chondrocytes were assessed by western blotting. The values are mean ± SD for 3 separate experiments. *p < 0.05, **p < 0.01 and ***p < 0.001. SDG, secoisolariciresinol diglucoside; ECM, extracellular matrix; IL-1β, interleukin-1β; COL2A1, collagen II; ADAMTS5, a disintegrin and metalloproteinase with thrombospondin motifs 5; SOX9, SRY-related high-mobility-group-box gene 9; MMP13, matrix metalloproteinases 13; INOS, inducible nitric oxide synthase; COX2, cyclooxygenase-2.

Article Snippet: Primary antibodies against Actin, COL2A1, ADAMTS5, SOX9, MMP13, INOS, COX2, Nrf2, HO-1 and Z. Zhang et al. Biomedicine & Pharmacotherapy 164 (2023) 114964 IκBαwere bought from Boster (Wuhan, China).

Techniques: Western Blot

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Secoisolariciresinol diglucoside Ameliorates Osteoarthritis via Nuclear factor-erythroid 2-related factor-2/ nuclear factor kappa B Pathway: In vitro and in vivo experiments.

doi: 10.1016/j.biopha.2023.114964

Figure Lengend Snippet: Fig. 7. The protective effects of SDG in IL-1β-activated SW1353 were reversed by Nrf2-siRNA. (A)The protein expressions of Nrf2 and HO-1 were evaluated by western blotting in chondrocytes transfected with Nrf2 siRNA. (B)The protein expressions of COL2A1, ADAMTS5, SOX9, MMP13, INOS and COX2 was assessed by western blotting in chondrocytes transfected with Nrf2 siRNA. The values are mean ± SD for 3 separate experiments. *p < 0.05, **p < 0.01 and ***p < 0.001. SDG, secoisolariciresinol diglucoside; IL-1β, interleukin-1β; Nrf2, Nuclear factor-erythroid 2-related factor-2; HO-1, Heme Oxygenase-1; COL2A1, collagen II; ADAMTS5, a disintegrin and metalloproteinase with thrombospondin motifs 5; SOX9, SRY-related high-mobility-group-box gene 9; MMP13, matrix metalloproteinases 13; INOS, inducible nitric oxide synthase; COX2, cyclooxygenase-2.

Article Snippet: Primary antibodies against Actin, COL2A1, ADAMTS5, SOX9, MMP13, INOS, COX2, Nrf2, HO-1 and Z. Zhang et al. Biomedicine & Pharmacotherapy 164 (2023) 114964 IκBαwere bought from Boster (Wuhan, China).

Techniques: Western Blot, Transfection

Journal:

Article Title: Regulation of Colonic Epithelial Repair in Mice by Toll-like Receptors and Hyaluronic Acid

doi: 10.1053/j.gastro.2009.08.055

Figure Lengend Snippet: (A) Wild type and MyD88−/− mice were given intraperitoneal HA at time 0 and plasma HA levels were measured at 12, 24, 48 and 72 hours. n=4, *p<.001 compared with MyD88−/− .. (B) Wild type mice were given intraperitoneal HA on days 1,3 and 5. The mice were sacrificed on day 7 and mRNA was extracted from the distal colon. RT-PCR was done for HAS-1, HAS-2, and HAS-3. n=4, *p<.01, **p<.001 compared with untreated. (C) TNFα production in the distal colon increased over 3 fold in WT mice treated with HA (30mg/kg), as measured by ELISA. Pretreating with PEP1 (40mg/kg) for 1 hour blocked HA induction of TNFα production. The data are the means +/− SE for 9 mice. *p<0.001 compared with HA treated mice. (D) IF for MIP2 (green) and F4/80 (red) in distal colons of WT mice shows that most of the MIP2 positive cells were macrophage as indicated by MIP2 colocalization (arrows) with the macrophage marker F4/80 (original magnification 400X). E. Western blot analysis shows that administration of HA induced 3 fold increases in the expression of COX-2 and MIP2 in the distal colons of WT but not MyD88−/− mice. PEP1 blocked the induction of COX2 and MIP2 by HA in WT but not MyD88−/− mice.

Article Snippet: Immunohistochemistry Primary antibodies were goat anti-mouse BrdU (1:100), mouse anti COX-2 (Pharmagen; 1:1000), goat anti-mouse MIP2 (R&D Systems,1:100), HAS-1, HAS-2 and HAS-3 (Santa Cruz, 1:100).

Techniques: Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Marker, Western Blot, Expressing

Journal:

Article Title: Regulation of Colonic Epithelial Repair in Mice by Toll-like Receptors and Hyaluronic Acid

doi: 10.1053/j.gastro.2009.08.055

Figure Lengend Snippet: Immunofluorescence for COX-2 (red) in the lamina propria of the distal colon of WT mice. Mice were untreated or received HA on days 1,3 and 5 or received HA and PEP1 on days 1,3 and 5. Mice were sacrificed on day 7. (original magnification 400X). B) The number of COX-2 expressing cells Data are the means +/−SE for 4 mice. * p< 0.001 comparing HA treated with untreated or HA+PEP1 treated mice. C) Immunofluorescence for COX-2 (red) and TLR4 (green) in mouse distal colons. Mice were untreated or treated with HA on days 1,3 and 5 and sacrificed on day 7. (original magnification 400X). D) Immunofluorescence for COX-2 (red) and F4/80 (green) in the colons of untreated and HA treated mice (Arrow: yellow cell with colocalization of COX-2 and F4/80).

Article Snippet: Immunohistochemistry Primary antibodies were goat anti-mouse BrdU (1:100), mouse anti COX-2 (Pharmagen; 1:1000), goat anti-mouse MIP2 (R&D Systems,1:100), HAS-1, HAS-2 and HAS-3 (Santa Cruz, 1:100).

Techniques: Immunofluorescence, Expressing

Journal:

Article Title: Regulation of Colonic Epithelial Repair in Mice by Toll-like Receptors and Hyaluronic Acid

doi: 10.1053/j.gastro.2009.08.055

Figure Lengend Snippet: HA treatment failed to rescue the DSS-induced weight loss and histologic score in COX2−/− mice. (A) Weight loss in COX2−/− mice. *p <0.01 compared with untreated. (B). Histologic scores in COX-2−/− mice. *p<0.001 compared with untreated.

Article Snippet: Immunohistochemistry Primary antibodies were goat anti-mouse BrdU (1:100), mouse anti COX-2 (Pharmagen; 1:1000), goat anti-mouse MIP2 (R&D Systems,1:100), HAS-1, HAS-2 and HAS-3 (Santa Cruz, 1:100).

Techniques: